Somatic Embryogenesis: Methods and Protocols

ISBN-13: 9781071624845 / Angielski / Twarda / 2022

Ramírez-Mosqueda, Marco A.

Opis

This volume provides an overview of methods for plant species, mainly species with high economic value, to achieve in vitro propagation through somatic embryogenesis. Chapters guide readers through somatic embryogenesis protocols, somatic embryos in plants, and new methodologies that are used out to solve in vitro recalcitrance of many species to this morphogenetic route. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Embryogenesis: Methods and Protocols aims to guide future researcher to achieve success in obtaining and regenerating somatic embryos. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

1. Overview of Somatic Embryogenesis

Marco A. Ramírez-Mosqueda

 

Part I: Somatic embryogenesis: advances in recalcitrant species

 

2. Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant

A. Mujib, Yashika Bansal, Moien Qadir Malik,  Rukaya Syeed, Jyoti Mamgain, and Bushra Ejaz

 

3. Advances in Somatic Embryogenesis in Vanilla (Vanilla planifolia Jacks.)

Marco A. Ramirez-Mosqueda, Jericó J. Jabín Bello-Bello, Arturo A. Armas S. Marco V. Rodríguez-Deméneghi, and Eduardo. Martínez-Santos

 

4. Somatic Embryogenesis in Pines

Ander Castander-Olarieta, Paloma Moncaleán, and Itziar A. Montalbán

 

Part II: Somatic embryogenesis in agroindustrial species: protocols

 

5. Use of thin cell layer technique for induction of somatic embryogenesis of Agave fourcroydes

Kelly M Monja-Mio, Antonio Rescalvo-Morales and Manuel L. Robert

 

6. Theobroma Cacao: Somatic Embryogenesis

Caroline Guillou and Dorothée Verdier

 

7. Somatic Embryogenesis in Sugarcane (Saccharum spp.)

Felipe Astolpho Almeida, Claudete Santa-Catarina, and Vanildo Silveira

 

8. Somatic embryogenesis in banana (Musa spp.)

Jorge López, Aymé Rayas, Víctor Medero, Arletys Santos, Milagros Basail and Yoel Beovides

 

9. Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis

Leonardo Soriano, Ahmad A Omar, and Adriana P Martinelli

 

10. Somatic embryogenesis in citrus (Citrus spp.), var. Valencia

Joanne Moraes de Melo Souza, Carolina Rossi de Oliveira, Eveline Carla da Rocha Tavano, Leonardo Soriano, and Adriana Pinheiro Martinelli

 

11. Somatic Embryogenesis from Immature Olive zygotic embryos

El Bahri Trabelsi and Amna Jedidi

 

12. Somatic embryogenesis in papaya (Carica papaya L.)

Laisyn Posada-Perez, Rafael Gómez-Kosky, Yenny Padron1, Diosdada Galvez, and Dion Daniels

 

Part III. Somatic embryogenesis in ornamental species: protocols

 

13. Somatic Embryogenesis of Anthurium andraeanum Linden. - A Tropical Florists’ Plant

T. K. Bandyopadhyay, C. Bhattacharya, S. Roy, P. Raha, I. Khatua, G. Saha and A. Chakraborty

 

Part IV: Strategies for obtaining somatic embryogenesis

 

14. Use of Thin Cell Layer (TCL) to obtain somatic embryogenesis

Ivonne N. Bravo-Ruiz, Ma. Teresa González-Arnao, Odón Castañeda-Castro, Miriam C. Pastelín-Solano, and Carlos A. Cruz-Cruz

 

15. Inorganic Compounds that Aid in Obtaining Somatic Embryos

Rodrigo Atanacio-López, Mauricio Luna-Rodríguez, Anell Soto-Contreras, Luz I. Rojas-Avelizapa, Nadia G. Sánchez-Coello, Norma Mora-Collado, and Rosalía Núñez-Pastrana

 

16. In vitro Stress-Mediated Somatic Embryogenesis in Plants

José Luis Spinoso-Castillo and Jericó Jabín Bello-Bello

 

Part V: Biotechnological Strategies for the Study and Use Of Somatic Embryogenesis

 

17. Protocol of Histological Procedures During Coconut Somatic Embryogenesis

Luis Sáenz Carbonell, Carlos Oropeza Salín, Ben Hur Chuc Armendariz, Alfonso Azpeitia Morales

18. Somatic Embryogenesis of Brachiaria brizantha (Syn. Urochloa brizantha) analyzed by in situ hybridization

Sylvia Rodrigues da Silveira, Andréa Dias Koehler, Ana Cristina M Mendes Gomes, Glaucia Barbosa Cabral, Vera Tavares de Campos Carneiro, Diva Maria de Alencar Dusi, and Adriana Pinheiro Martinelli

 

Part VI: General Conclusions and Perspectives

 

19. Perspectives of Somatic Embryogenesis: Concluding Remarks

Marco A. Ramírez-Mosqueda