Single-Cell Analysis: Methods and Protocols

ISBN-13: 9781617795664 / Angielski / Twarda / 2012 / 238 str.

Sara Lindstr M.; Helene Andersson-Svahn

Opis

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail. The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.

Spis treści

1. Introduction: Why Analyze Single Cells?

Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett

 

Part I: Conventional Methods

 

2. Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview

            Sara Lindström

 

3. Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry

            Robert B. Brown, Johannes A. Hewel, Andrew Emili, and Julie Audet

 

4. Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts

            Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom

 

Part II: Miniaturized Solutions

 

5. Single-Cell Culture in Microwells

            Sara Lindström and Helene Andersson-Svahn

 

6. Iso-Dielectric Separation and Analysis of Cells

            Michael D. Vahey and Joel Voldman

 

7. Single Cell Electroporation Using Microfluidic Devices

            Séverine Le Gac and Albert van den Berg

 

8. Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array

            Chi Zhang

 

9. Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay

            Sara Henriksson and Mats Nilsson

 

10. Droplet Microfluidics for Single-Cell Analysis

            Eric Brouzes

 

11. Screening of Antigen-Specific Antibody Secreting Cells

            Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, and Atsushi Muraguchi

 

12. Analysis of Single Eukaryotic Cells Using Raman Tweezers

            Elsa Correia Faria and Peter Gardner

 

13. Single-Cell Microinjection Technologies

            Yan Zhang

 

14. Ultrasonic Manipulation of Single Cells

            Martin Wiklund and Björn Önfelt

 

Part III: Reviews on Chosen Subjects Within the Field

 

15. Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices

            Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii

 

16. Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses

            Ayça Yalçın, Yvonne J. Yamanaka, and J. Christopher Love