reporter genes: a practical guide

ISBN-13: 9781588297396 / Angielski / Twarda / 2007 / 172 str.

Anson, Don

Opis

Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.

Spis treści

Table of contents Preface. Donald S. Anson. 1. Methodologies for staining and visualisation of '-galactosidase in embryos and tissues. Siobhan Loughna* and Deborah Henderson. 2. Immunohistochemical Detection of Beta-Galactosidase or Green Fluorescence Protein on Tissue Sections. Philip A. Seymour and Maike Sander*. 3. Detection of Reporter Gene Expression in Murine Airways. Maria Limberis*, Peter Bell, and James M. Wilson. 4. Three dimensional analysis of molecular signals with episcopic imaging techniques. Wolfgang J. Weninger* & Timothy J. Mohun 5. Fluorescent proteins for cell biology. George H. Patterson* 6. Detection of GFP during nervous system development in Drosophila melanogaster. Karin Edoff, James S. Dods and Andrea H. Brand* 7. Autofluorescent Proteins for Flow Cytometry. Charles G. Bailey and John E. J. Rasko* 8. Fluorescent protein reporter systems for single cell measurements. Steven K. Dower*, Eva E. Qwarnstrom and Endre Kiss-Toth 9. Subcellular Imaging of cancer cells in live mice. Robert M. Hoffman* 10. Non-invasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects. Pritha Ray and Sanjiv Sam Gambhir*. 11. Green Fluorescent Protein as a Tracer in Chimeric Tissues. Harald Jockusch* and Daniel Eberhard

Blurb

Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.