Recombinant Virus Vaccines: Methods and Protocols

ISBN-13: 9781493968671 / Angielski / Twarda / 2017 / 273 str.

Gary R. Skuse; Maureen C. Ferran

Opis

This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms. Recombinant Virus Vaccines: Methods and Protocols details the use of recombinant vaccines that are employed to either produce immunogens in vitro or elicit antibody production in vivo. The chapters in this book are divided into four parts: Part I explores double-stranded DNA viruses; Part II discusses negative sense single-stranded RNA viruses; Part III talks about positive sense single-stranded RNA viruses; and Part IV describes bacteriophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Recombinant Virus Vaccines: Methods and Protocols is a valuable resource for scientists and clinicians who are interested in learning about and adopting methods for use in basic and biomedical research directed toward generating and developing recombinant viral vaccines.

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Preface…
Table of Contents…
Contributing Authors…

Part I Double-Stranded DNA Viruses     

1.   Development of Novel Vaccines Against Infectious Diseases Based on Chimpanzee Adenoviral Vector
Chao Zhang, Yudan Chi, and Dongming Zhou

2.   Development of Recombinant Canarypox Viruses Expressing Immunogens
Débora Garanzini, María Paula Del Médico-Zajac, and Gabriela Calamante

3.   Fowl Adenovirus-Based Vaccine Platform
Juan C. Corredor, Yanlong Pei, and Éva Nagy

4.   Development of Recombinant HSV-Based Vaccine Vectors
Richard Voellmy and David Bloom

5.    Generating Recombinant Pseudorabies Virus for Use as a Vaccine Platform
Feifei Tan, Xiangdong Li, and Kegong Tian

6.    Generation and Production of Modified Vaccinia Virus Ankara (MVA) as a Vaccine Vector
Vincent Pavot, Sarah Sebastian, Alison V. Turner, Jake Matthews, and Sarah C. Gilbert

7.    Poxvirus Safety Analysis in the Pregnant Mouse Model, Vaccinia and Raccoonpox Viruses
Rachel L. Roper

Part II Negative Sense Single-Stranded RNA viruses      

8.    Development of Recombinant Arenavirus-Based Vaccines
Luis Martínez-Sobrido and Juan Carlos de la Torre

9.    Development of Recombinant Measles Virus-Based Vaccines
Michael D. Mühlebach and Stefan Hutzler

10. Recombinant Tri-Segmented Pichinde Virus as a Novel Live Viral Vaccine Platform
Rekha Dhanwani, Hinh Ly, and Yuying Liang

11. Human Rhinovirus-A1 as an Expression Vector
Khamis Tomusange, Danushka Wijesundara, Eric James Gowans, and Branka Grubor-Bauk

12. Generating Recombinant Vesicular Stomatitis Viruses for Use as Vaccine Platforms
John B. Ruedas and John H. Connor

Part III Positive Sense Single-Stranded RNA viruses       

13. Alphavirus-Based Vaccines
Kenneth Lundstrom

Part IV Bacteriophage   

14. Display of HIV-1 Envelope Protein on Lambda Phage Scaffold as a Vaccine Platform
Jonelle L. Mattiacio, Matt Brewer, and Stephen Dewhurst

15. Bacteriophage T4 as a Nanoparticle Platform to Display and Deliver Pathogen Antigens: Construction of an Effective Anthrax Vaccine
Pan Tao, Qin Li, Sathish Shivachandra, and Venigalla B. Rao