Production of serine protease from sporotrichum thermophile

ISBN-13: 9783659907609 / Angielski / Miękka / 2016 / 60 str.

Sikander Ali

Opis

In the present study, Sporotrichum thermophile ATCC 42464 was used for serine protease production using solid substrate fermentation (SSF). Wheat bran (12.5 g) was employed as the carbon source. Cultural conditions such as moisture content (MSS-12.5 ml), pH (5) and time of incubation (48 h) were optimized. The enzyme activity decreased in the presence of inhibitors (urea, SDS) and chelators (EDTA) while presence of metal ions especially Mg2+ caused an increase in the activity of serine protease. The maximal enzyme activity of 18.84 U/g was observed under the conditions optimized.

O autorze

Dr. Sikander Ali did his Ph.D (2005) from University of the Punjab, Lahore. He is an eminent researcher in Fermentation Technology and one of the founder members who established Institute of Industrial Biotechnology at GC University Lahore. He has several international research publications to his credit. Currently, he is an 'Associate Professor'.