Plant Secondary Metabolism Engineering: Methods and Protocols

ISBN-13: 9781071621844 / Angielski / Twarda / 2022

Fett-Neto, Arthur Germano

Opis

This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field. 

Spis treści

1. Extraction and Analysis of Phenolic Compounds From Grape Berries

Kamal Tyagi, Larry Lerno, Mirko De Rosso, Itay Maoz,  Amnon Lichter, Susan E. Ebeler, and Riccardo Flamini

 

2. Construction of the UDP-glucose biosynthetic Enzyme Gene Co-Expression Plasmid For Prunasin Production in Escherichia coli

Takuya Yamaguchi and Yasuhisa Asano

 

3. Histochemical Localization of Phenolic Compounds And Reactive Oxygen Species In Eucalypt Microcuttings

Johnatan Vilasboa, Cibele Tesser da Costa, Jorge Ernesto de Araújo Mariath, and Arthur Germano Fett-Neto

 

4. A Bioinformatics Tool for Efficient Retrieval of High-Confidence Terpene Synthases (TPS) and Application to the Identification of TPS in Coffea and Quillaja

Douglas S. Domingues, Liliane S. Oliveira, Samara M. C. Lemos,  Gian C. C. Barros,  and Suzana T. Ivamoto-Suzuki

 

5. Use of Catharanthus roseus Cell Cultures for the Synthesis Of Metal Nanoparticles

Dandy Ahamefula Osibe and Hideki Aoyagi

 

6. Fungal Pigment Assisted Silver Nanoparticle Synthesis and their Antimicrobial and Cytotoxic Potential

Sharad Bhatnagar, Toshiro Kobori, Deepak Ganesh, and Hideki Aoyagi

 

7. Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses

Fernanda Salvato, Raquel Figueiredo, and Paulo Mazzafera

 

8. Visualization of Suberization and Lignification in Sugarcane

Raquel Figueiredo, Juan Pablo Portilla Llerena, Bárbara Rocha Cardeli, and Paulo Mazzafera

 

9. Isolation of Promoters and Transcription Factors Involved In The Regulation of Lignin Biosynthesis in Saccharum species

Juan Pablo Portilla Llerena, Raquel Figueiredo, Sávio Siqueira Ferreira, Igor Cesarino, and Paulo Mazzafera

 

10. Soapbark Triterpenes: Quillaja brasiliensis Cell Culture Sapogenin and Free Sterol Analysis by GCMS

Yve V. S. Magedans and Michael A. Phillips

 

11. Generation of stable Catharanthus roseus hairy root lines with Agrobacterium rhizogenes

Krystyna K. F. Traverse, Samuel Mortensen, Juliet G. Trautman, Hope Danison, Noreen F. Rizvi, and Carolyn W.T. Lee-Parsons

12. Analyzing the Distribution of Specialized Metabolites From Plant Native Tissues With Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging

Abigail Moreno-Pedraza, Nancy Shyrley Garcia-Rojas and Robert Winkler

 

13. Virus-Induced Gene Silencing as a Tool to Study Regulation of Alkaloid Biosynthesis in Medicinal Plants

Barunava Patra, Yongliang Liu, Joshua J. Singleton, Sanjay Kumar Singh, and Sitakanta Pattanaik, and Ling Yuan

 

14. Cyclotide isolation from Psychotria brachyceras and Psychotria leiocarpa

Hélio Nitta Matsuura

 

15. Formulation of IMXQB: nanoparticles based on Quillaja brasiliensis saponins to be used as vaccine adjuvants

Mariana Rivera-Patron, Samuel P. Cibulski, Iris Miraballes,  and Fernando Silveira

 

16. Isolation of specialized plant cells by fluorescence-activated cell sorting

Joana G. Guedes, Ana Luísa Guimarães, Inês Carqueijeiro, Rui Gardner, Cláudia Bispo and Mariana Sottomayor

 

17. X-Ray Computed Tomography for 3D Anatomical Analysis of Resin Ducts

Irenka Fabián-Plesníková, Franciele Antônia Neis, and Adolpho Herbert Augustin

 

18. Untargeted metabolomic analysis of non-volatile and volatile glucosinolates in Brassicaceae

Yu LIU, Li ZOU, and Choon Nam ONG

 

19. Methods of mimosine extraction from Leucaena leucocephala (Lam.) de Wit leaves

Kelly Cristine da Silva Rodrigues-Corrêaa, Michael David Hideo Hondab, Dulal Borthakurb and Arthur G. Fett-Netoa

  20. Microbial production, extraction, and quantitative analysis of isoprenoids

Alessandro Satta, Zeyu Lu, Manuel R. Plan, Lygie Esquirol, and Birgitta E. Ebert