Oligodendrocytes: Methods and Protocols

ISBN-13: 9781493990702 / Angielski / Twarda / 2019 / 411 str.

David Lyons; Linde Kegel

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System

Sueli Marques, David van Bruggen, and Gonçalo Castelo-Branco

2. Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing

Claire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, and Veronique E. Miron

3. Myelin: Methods for Purification and Proteome Analysis

Michelle S. Erwig, Dörte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer, Olaf Jahn, and Hauke B. Werner

4. In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells

Dario Magnani, Siddharthan Chandran, David J.A. Wyllie, and Matthew R. Livesey

5. Isolation and Culture of Oligodendrocytes

Marie-Theres Weil, Giselheid Schulz-Ëberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schäfer, Christina Müller, and Mikael Simons

6. A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation

Marie E. Bechler

7. Oligodendrocyte-Neuron Myelinating Co-Culture

Matthew Swire and Chalres ffrench-Constant

8. Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier

Andrew A. Jarjour and Diane L. Sherman

9. Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices

Sylvia Agathou and Ragnhildur Thóra Káradóttir

10. Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord

Sowmya Sekiza and Anna Williams

11. Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination

Linde Kegel, Maria Rubio, Rafael Almeida, Silvia Benito, Anna Klingseisen, and David A. Lyons

12. Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination

Jill M. Williamson, David A. Lyons, and Rafael G. Almeida

13. A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation

Marja J. Karttunen and David A. Lyons

14. Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis

Abdelkrim Mannioui and Barnard zalc

15. Conditional Mutagenesis in Oligodendrocyte Lineage Cells

Sandra Goebbels and Klaus-Armin Nave

16. Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage

Jaime Eugenin-von Bernhardi and Leda Dimou

17. The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination

Maria Traka

18. Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease

John N. Mariani, Lisa Zou, and Steven A. Goldman

19. A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes

Graham R. Campbell and Don J. Mahad

20. Transmission Electron Microscopy of Oligodendrocytes and Myelin

Marie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Mogusia Sadowski, and Wiebke Möbius

21. Toxin-Based Models to Investigate Demyelination and Remyelination

Christopher E. McMurran, Chao Zhao, and Robin J M. Franklin

22. Magnetic Resonance techniques for Imaging White Matter

Cassandra Sampaio-Baptista, Kata Diosi, and Heidi Johansen-Berg

Blurb

This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.