Modern Clinical Molecular Techniques

ISBN-13: 9781461421696 / Angielski / Twarda / 2012 / 436 str.

Peter Hu; Madhuri Hegde; Patrick Alan Lennon

Opis

This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice.Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory."

Spis treści

Preface

Acknowledgement

Contributors

 

 

Section I   Current Molecular Laboratory Methods

 

1        Specimen Collection, Handling and Processing

Lindsy Hengesbach and John Gerlach

 

2        DNA/RNA Isolation and Quantitation

Jing Wang

 

3        Test Validation

Cindy McCloskey and S Terence Dunn

 

4        In Vitro Amplification Methods in Molecular Diagnostics

C. Alexander Valencia and Bradford Coffee

 

5        Whole Genome Sequencing in the Clinical Laboratory

Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter

 

6        Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction

Juliana Purrazzella and Arupa Ganguly

 

7        Reporting Clinical Molecular Genetic Laboratory Results

Victor Wei Zhang and Lora J. H. Bean

 

 

Section II Clinical Molecular Infectious Disease Testing

 

8        HIV-1 Genotyping and Virtual Phenotyping

Fangling Xu and Jianli Dong

 

9        COBAS® Ampliprep/Taqman HIV-1 Test

Tavat A. Buraruk and Charles E. Stager

 

10    Hepatitis C Virus (HCV) Genotype Assay (LiPA)

Charles E. Stager and Tavat A. Buraruk

 

11    COBAS® Ampliprep/Taqman HCV Test

Charles E. Stager and Tavat A. Buraruk

 

12    Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction

Su S. Chen, Seema Hai, and Rajyalakshmi Luthra

 

13    Quantification of BK Viral Load

Hai Wu and Jianli Dong

 

14    Molecular Diagnosis and Epidemiology of Rabies

Rodney E. Rohde and Bonny C. Mayes

 

15    Pyrosequencing for Bacterial Identification

Laura Millecker and Ruth Ann Luna

 

16    Other Pathogens of Significant Public Health Concern

Chris L. McGowin, Rodney E. Rohde, and Greg C. Whitlock

 

 

 

Section III Clinical Molecular Oncology Testing

 

17    Gene Rearrangements Testing

Siby Sebastian, Juan Du, and Rizwan Naeem

 

18    Mutation Analysis in Cancer

Keyur Pravinchandra Patel, John Galbincea,  and Rajyalakshmi Luthra

 

19    Minimal Residual Disease

Wesley Greaves and Rajyalakshmi Luthra

 

20    Pyrosequencing in Cancer

Bedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma

 

21    Clinical Applications of Microarrays in Cancer

Marilyn M. Li, Ankita Patel, and Xiaofeng Hu

 

22    Basic Theories and Clinical Applications of Molecular Flow Cytometry

Hua Kang Wu, Hongli Chen, and Peter Hu

 

 

Section IV Clinical Molecular Pre/Postnatal Testing

 

23    Molecular Prenatal Diagnostic Testing

Joanna Wiszniewska and Christine M. Eng

 

24    PCR Based Diagnosis of Fragile X Syndrome 

Daniel J. Dykas and Allen E. Bale

 

 

Section V Human Identity Testing

 

25    Microsatellite Testing Short Tandem Repeat Profile Generation

Robin DeVille Guidry and Laura Gahn

 

26    Parentage

Laura Gahn and Robin DeVille Guidry

 

 

Section VI Clinical Molecular Training

 

27    Diagnostic Molecular Training

Peter Hu, Patrick A. Lennon, and Madhuri Hegde

 

28    Molecular Diagnostics Point of Care Testing Training

Chang W. Lee, and Peter Hu