Macrophages: Methods and Protocols

ISBN-13: 9781493978366 / Angielski / Twarda / 2018 / 278 str.

Germain Rousselet

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Culture of Human Monocyte-Derived Macrophages

Aoife Kelly, Aleksander M. Grabiec, and Mark A. Travis

2. A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages

Chandrayana Mukherjee, Christine Hale, and  Subhankar Mukhopadhyay

3. Growing Murine Bone Marrow-Derived Macrophages

Anaïs Assouvie, Lisa P. Daley-Bauer, and Germain Rousselet

4. Immortalization of Murine Bone Marrow-Derived Macrophages

Dominic De Nardo, Dhan V. Kalvakolanu, and Eicke Latz

5. Isolation and Identification of Murine Serous Cavity Macrophages

Calum C. Bain and Stephen J. Jenkins

6. Isolation and Identification of Interstitial Macrophages from the Lungs using Different Digestion Enzymes and Staining Strategies

Shaikh M. Atif, Sophie L. Gibbings, and Claudia V. Jakubzick

7. Isolation and Phenotyping of Adult Mouse Microglial Cells

Kathleen Grabert and Barry W. McColl

8. Isolation and Phenotyping of Bone Marrow Macrophages

Marion Chalot

9. Isolation and Phenotyping of Intestinal Macrophages

Vanessa Petit

10. Phenoytyping Tumor Associated Macrophages

R. Ian Cumming and Yen-Rei A. Yu

11. Activating Murine Macrophages In Vitro

Elizabeth Dalby

12. Polarizing Macrophages In Vitro

Xuan Huang, Yong Li, Mingui Fu, and Hong-Bo Xin

13. Viral Replication Assay in Bone Marrow-Derived Macrophages

Linda Roback and Lisa P. Daley-Bauer

14. Macrophage Bactericidal Assays

Mourad Aribi

15. Quantitative Phagocytosis Assays in Primary and Cultured Macrophages

Fernando Montaño, Sergio Grinstein, and Roni Levin

16. Observing Frustrated Phagocytosis and Phagosome Formation and Closure using Total Internal Reflection Fluorescence Microscopy (TIRFM)

Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, and Florence Niedergang

17. Chromatine Immunoprecipitation in Macrophages

Germain Rousselet

18. Transfecting Macrophages

Andrea-Anneliese Keller, Marten B. Maeß, Michael Schnoor, Berith Wittig, and Stefan Lorkowski

19. In Vitro Migration Assays

Lewis Taylor, Carlota Recio, David R. Greaves, and Asif J. Iqbal

20. Cytokine-Induced Acute Inflammatory Monarticular Arthritis

Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, and Andrew D. Cook

21. Characterizing Activation, Proliferation, and Ontogeny of Murine Macrophages in Parasitic Helminth Infections

Dominik Ruckerl

22. Genetic Models of Macrophage Depletion

Li Hua, Jiayuan Shi, Leonard D. Shultz, and Guangwen Ren

23. Depleting Macrophages In Vivo with Clodronate-Liposomes

Stéphanie G. Moreno

24. Cre Driver Mice Targeting Macrophages

Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, and Guangwen Ren

Blurb

This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.

The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.