Light and Video Microscopy

ISBN-13: 9780124114845 / Angielski / Twarda / 2014 / 350 str.

Wayne, Randy O.

Opis

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.

• Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope
• Clearly develops all ideas from historical and logical foundations
• Laboratory exercises included to assist the reader with practical applications
• Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

Recenzje

"As such, this book would be an excellent undergraduate-level text on optical microscopy for biologists. It is also valuable to anyone using a light microscope." --Carol Bayles, Cornell University, Ithaca, NY, USA

Spis treści

Chapter 1: The Relation Between the Object and the ImageChapter 2: Geometrical OpticsChapter 3: Physical OpticsChapter 4: Bright Field MicroscopyChapter 5: Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique IlluminationChapter 6: PhotomicrographyChapter 7: Fluorescence MicroscopyChapter 8: Polarization MicroscopyChapter 9: Interference MicroscopyChapter 10: Differential Interference MicroscopyChapter 11: Modulation Contrast MicroscopyChapter 12: Video and Digital MicroscopyChapter 13: Image Processing and AnalysisChapter 14: Various Kinds of Microscopes and Accessories

O autorze

Randy O. Wayne is a plant cell biologist at Cornell University notable for his work on plant development. In particular, along with his colleague Peter K. Hepler, Wayne established the powerful role of calcium in regulating plant growth; accordingly, their 1985 article, Calcium and plant development, was cited by at least 405 subsequent articles to earn the "Citation Classic" award from Current Contents magazine and has been cited by hundreds more since 1993. He is an authority on how plant cells sense gravity through pressure, on the water permeability of plant membranes, light microscopy, as well as the effects of calcium on plant development. He has published over 50 articles and is the author of another book, Light and Video Microscopy.