Kategoria BISAC: Nature >> Animals - Mammals

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,...

The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns...

Nuclear Receptors focuses on the structural analysis of nuclear receptors from the initial work using isolated protein domains to the more recent exciting developments investigating the conformational shape of full-length receptor complexes. The book also reviews the structure of key nuclear receptor co-regulatory proteins. It brings together, for the first time, a comprehensive review of nuclear receptor structure and the importance of receptor conformation underpinning allosteric regulation by different ligands (hormone, drugs, DNA response elements, protein-protein interactions)...

In 1992, in a remote mountain range, a team of scientists discovered the remains of an unusual animal with exquisite long horns. It turned out to be a living species new to Western science--a saola, the first large land mammal discovered in fifty years.

Rare then and rarer now, a live saola had never been glimpsed by a Westerner in the wild when Pulitzer Prize finalist and nature writer William deBuys and conservation biologist William Robichaud set off to search...

This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation...

• Represents the most comprehensive coverage of all sub-disciplines of dental anthropology available today
• Features individual chapters written by experts in their specific area of dental research
• Includes authors who also present results from their research through case studies or voiced opinions about their work
• Offers...

This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regulation, various expression profiling approaches, protein-RNA interaction and non-coding RNA investigation techniques, methods for profiling RNA modifications, and other techniques such as alternative translation initiation or polyadenylation sites determination. Written for the Methods...

This volume provides acollection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Yeast Functional Genomics: Methods...

Among all the large whales on Earth, the most unusual and least studied is the narwhal, the northernmost whale on the planet and the one most threatened by global warming. Narwhals thrive in the fjords and inlets of northern Canada and Greenland. These elusive whales, whose long tusks were the stuff of medieval European myths and Inuit legends, are uniquely adapted to the Arctic ecosystem and are able to dive below thick sheets of ice to depths of up to 1,500 meters in search of their prey-halibut, cod, and squid.

Join Todd McLeish as he travels high above the Arctic circle to...

Fully integrative approach to the socibiology of caviomorph rodents

• Brings together research on social systems with that on epigenetic, neurendocrine and developmental mechanisms of social behavior
  
  
  
• Describes the social systems of many previously understudied caviomorph species, identifying the fitness costs and benefits of social living in current day populations as well as quantified evolutionary patterns or trends

• Highlights potential parallels and differences with other animal models