This book constitutes two challenges that were held in conjunction with the 25th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2022, which took place in Singapore during September 18-22, 2022. The peer-reviewed 20 long and 5 short papers included in this volume stem from the following three biomedical image analysis challenges: Mitosis Domain Generalization Challenge (MIDOG 2022), Diabetic Retinopathy Analysis Challenge (CRAC 2022) The challenges share the need for developing and...