Protein microarrays have shown a powerful tool to identify cancer biomarkers due to the multiplex detection and tiny sample requirement. However, there is no single biomarker that is sensitive and specific enough to meet strict diagnostic criteria due to the diversity and variation of cancers. To this end, a combination of biomarkers is required to increase sensitivity and specificity. In this book, various silane monolayers and polymer layers were developed to functionalize 3D micro-structured glass slides to effectively immobilize proteins, and the detection parameters were optimized for...