This book contains lecture notes from most of the courses presented at the 50th anniversary edition of the Seminaire de Mathematiques Superieure in Montreal. This 2011 summer school was devoted to the analysis and geometry of metric measure spaces, and featured much interplay between this subject and the emergent topic of optimal transportation. In recent decades, metric measure spaces have emerged as a fruitful source of mathematical questions in their own right, and as indispensable tools for addressing classical problems in geometry, topology, dynamical systems, and partial differential...