Current study focused on the findings of nutritional conposition (oil, protein,carbohydrates, ash, moisture and fibre contents) and 13 mineral contents including N, Fe,Cu,Zn,Mg, Ca, etc., all were found in varying amount. Chromatographic techniques TLC, GLC and HPLC displayed differences in of the germplasm. The generic lines were identified that have the potential to be used in future hybridization and breeding programmes to develop high oil as well as rich nutrient lines. The results revealed that Nigella sativa L. needs further consideration as potential constant source of herbal,...