Antibody production is now a rapidly developing area where the uses of polyclonal antibodies are finding wide application. This book explains the antibody production starting from antigen preparation to anti-sera purification including protein dialysis and affinity chromatography. It also contains protocols for coating of anti-sera to solid phase and most buffers needed for polyclonal antibody production. It offers the basic principle of immunoassays.

We monitored post-treatment Plasmodium falciparum among patients treated with chloroquine (CQ) and Fansidar (SP) in a village in eastern Sudan. Parasites were examined on day0, day7, day14 and day21 during the transmission season. A further sample was taken 2 months later (day80) at the start of the dry season. Asexual forms and gametocytes were detected by microscopy, and reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect expression of gametocyte-specific proteins pfs25 and pfg377. Microscopic gametocytes increased significantly following CQ and SP treatment,...