The study is designed to develop genetic modification approach in methylotrophic bacteria through an effective expression system to enhance the ability of bioremediating bacteria to consume oil in polluted areas. The identification of bacterial isolates done based on morphological traits and the gene 16S rRNA. All analyzed sequences had an expected (E) value of 0, and more than 99% of the reported valid genera are very closely related to the BLASTN identity percentage and identified as Methylorubrum extorquens and Pseudomonas balearica. The plasmid-treated CRISPR-Cas9 system was used in M....