Le lit artériel mésentérique (LAM) est capable de sécréter certaines protéases solubles qui s'accumulent dans le liquide de perfusion. Notre laboratoire s'est consacré à la caractérisation de ces enzymes, parmi lesquelles l'élastase-2, précédemment décrite comme une enzyme uniquement digestive, et désormais également présentée comme la principale enzyme formant l'Ang II dans le perfusat du LAM de rat. Outre cette endopeptidase, des activités de carboxypeptidases ont été détectées dans ce perfusat, à l'aide de substrats synthétiques et de vasopeptides, tels que le ZVF,...

The mesenteric arterial bed (MAB) is capable of secreting some soluble proteases that accumulate in the perfusion fluid. Our laboratory has been dedicated to characterizing these enzymes, among them elastase-2, previously described as a solely digestive enzyme, and now also presented as the main enzyme forming Ang II in rat MAB perfusate. In addition to this endopeptidase, carboxypeptidase activities were detected in this perfusate using synthetic substrates and vasopeptides such as ZVF, Ang I, and Bk. The des-Arg9-Bk-forming activity was recently characterized as CPB1. Once again, a protease...

Das mesenteriale Arterienbett (LAM) ist in der Lage, einige lösliche Proteasen zu sekretieren, die sich in der Perfusionsflüssigkeit ansammeln. Unser Labor hat sich der Charakterisierung dieser Enzyme gewidmet, darunter Elastase-2, die zuvor als ausschließlich verdauungsförderndes Enzym beschrieben wurde und nun auch als das wichtigste Enzym zur Bildung von Ang II im Perfusat des LAM von Ratten vorgestellt wird. Neben dieser Endopeptidase wurden in diesem Perfusat Carboxypeptidase-Aktivitäten nachgewiesen, wobei synthetische Substrate und Vasopeptide wie ZVF, Ang I und Bk verwendet...

Thalassophryne nattereri is a small venomous fish that inhabits virtually the entire Brazilian coastline. It is a relatively frequent cause of accidents among fishing workers in the North and Northeast regions of Brazil, as well as among swimmers. Its venom causes an intense inflammatory reaction, followed by necrosis of the affected area, which persists for several days. As is usually the case with venoms, that of T. nattereri is a serous secretion, i.e., rich in proteins. Among these are several proteases and peptides, which have complex effects on the human body. The aim is to investigate...