The book contains basic knowledge of different biotechnological tools and techniques used nowadays for various purposed like genetic engineering, DNA fingerprinting, vaccine preparation, detection of viral, bacterial and protozoal infection etc. It provides valuable knowledge to the young researchers/students. This book is basically primed for the students of B.Sc and M.Sc level. We hope that this book will be helpful to the reader. Thankfully: - J Sharma, M Soni & A Patha