Analytical method development and validation play a major role in the discovery, development and manufacturing of pharmaceuticals. The official test method that results from these processes is used by quality control laboratories to ensure the identity, purity, potency and performance of drug product quality, essential for drug safety and efficiency. Method development is a formalized process by which a set of separation criteria are determined with a defined set of parameters such that the same separation profile is achieved. The process of method development can be quantitative or...

Charge transfer complexation between Rabeprazole and 2,3-dichloro-5,6-dicyano -1,4-benzoquinone (DDQ) as an acceptor has been investigated spectrophotometrically. Based on Job s method of continuous variation plots, the obtained results indicate the formation of 1:1 Charge transfer complex with general formula [(RA) (DDQ)] and Analyses carried out using literature data for backbone 15N NMR relaxation of 20 proteins that differ widely in their sequence, structure, and function show that the segments with defined secondary structural elements are generally stiff relative to less structured or...