This book presents a comprehensive guide to biotechnological approaches for producing bioactive compounds from tissue-cultured plants. It systematically covers: (1) fundamental principles of plant tissue culture and its advantages over wild harvesting; (2) optimized protocols for establishing cultures, including explant selection, media preparation, and yield enhancement strategies; (3) modern extraction techniques (solvent-based, supercritical fluid, ultrasound/microwave-assisted); (4) advanced analytical methods (HPLC, GC-MS, NMR, FTIR) and bioactivity assays; and (5) current challenges and...