Healthcare-associated infections (HAIs) represent a major public health problem worldwide. The objectives of our work were to estimate the prevalence of HAIs as well as the frequency of antibiotic use at the Charles Nicolle Hospital (HCN) in Tunis.This was a cross-sectional study at the HCN (October-December 2018). All patients present in the departments for more than 48 hours were included. Only active HAIs confirmed clinically and/or microbiologically were retained.In total, 318 patients were included with an average age of adult patients ( 18 years) of 52.3 years. The prevalence of HAI was...