The study conducted Line x Tester analysis using three female lines and eight male testers to assess heterosis, combining ability, and gene action in cotton. The experimental setup included eleven parents, resulting in twenty-four crosses and the standard check GN.Cot.Hy-14. Thirty-six genotypes were planted in a randomized block design with three replications at the Main Cotton Research Station, NAU, Surat during Kharif 2017. Analysis of variance revealed significant differences among parents and hybrids for various traits, indicating considerable genetic variability. Notably, certain...