Four epiphytic orchids viz., Dendrobium aphyllum, Vanda roxburghii, Rhynchostylis retusa, Luisia teretifolia collected from four places (Khulna, Coxs Bazar, Sylhet and Mymensingh) showed significant variation in different morphological and reproductive characters. The seeds of different species upon culture on to growth regulator free media were able to germinate asymbiotically and subsequent development into plantlet. Mature explants such as shoot tips, axilliary buds, leaf segments, flower stalk segments and root tips were also cultured in various types of culture media formulations....