Shorttitle: Geographical speciation occurs among geographically separated populations and is a common mechanism of speciation in a wide range of organisms. The use of modern genetic techniques has substantially improved our understanding of the identification and detection of the speciation process. Plasmodium vivax isolates inhabiting the Old World and New World regions so far, were considered to be as two different subspecies or separate species because of the variant transmission phenotypes and differences in nuclear and plastid genetic markers. We explored a panel of novel and known...