Serum autoantibody (auto-Ab) testing is useful for diagnosing autoimmune hepatopathies (AIH). This test is performed by indirect immunofluorescence (IFI) on triple substrate, and may be supplemented by other techniques. Among 167 requests for testing for auto-Ac associated with HPAI, triple substrate IFI was positive in 19 cases (11.4%), with anti-mitochondria Ac detected in 14 cases (8.4%) and anti-smooth muscle Ac in 5 cases (3%). IFI on VSM47 identified anti-actin F antibodies in 3/5 cases. Immunodot confirmed the positivity of anti-mitochondria Ac in all 14 cases detected by IFI, with or...

Der Serumnachweis von Autoantikörpern (Auto-AK) ist bei der Diagnose von Autoimmunhepatopathien (AIHP) hilfreich. Dieser Nachweis erfolgt mittels indirekter Immunfluoreszenz (IFI) auf Dreifachsubstrat und kann durch andere Techniken ergänzt werden. Von den 167 Anträgen auf Suche nach Auto-Ak im Zusammenhang mit HPAI war die IFI auf Triple-Substrat in 19 Fällen (11,4%) positiv, wobei in 14 Fällen (8,4%) Anti-Mitochondrien-Ak und in 5 Fällen (3%) Anti-Glatte-Muskel-Ak nachgewiesen wurden. Der IFI auf VSM47 identifizierte Anti-F-Actin-Aktien in 3/5 Fällen. Der Immunodot bestätigte die...

Neuromyelitis optica spectrum disorders (NMOSD) are a rare group of inflammatory demyelinating diseases of the central nervous system whose etiopathogenesis involves genetic factors. The association between HLA and NMOSD has been studied in various populations. However, no association study has been conducted in Tunisia.We therefore conducted a case-control study including 12 NMOSD patients (recruited over a period of 11 years) and 123 unrelated healthy subjects from the South Tunisian population. HLA class I typing was performed using the complement-dependent lymphocytotoxicity technique....

Monitoring of patients treated for differentiated thyroid cancer (DTC) includes serum thyroglobulin (TG) and anti-TG antibodies (Ac). The aim of our study was to describe the methodology and interpret the results of these two parameters.TG and anti-TG Ac assays were performed by electro-chimiluminescence and ELISA, respectively.Thirty-five patients were included. TG was undetectable in 18 cases and detectable in 17 (0.12 to 51.32 ng/ml). The TG result was not interpretable in 2 cases due to the positivity of anti-TG Ac (>100 IU/ml). In the absence of anti-TG Ac (33 cases), the TG result was...