The motivation of this book is to develop the merits of infrared spectroscopic based methods that could be used as a detector/identifier in biological detection systems. The methods described in this book are based on Attenuated Total Reflection (ATR) because of the requirement of detection of spores in water. The use of ATR requires concentrating the spores onto the surface of internal reflection elements (IRE). In chapter 3, an electrostatic method for concentrating Bacillus globigii spores on the ZnSe IRE with alumina coating is presented. In chapter 4, an electric field assisted ATR was...