Studies on the prevalence of the Extended Spectrum Beta Lactamase Enzyme Producing Enterobacteriaceae Group of Bacterial isolates from Bovine Milk were undertaken in the 100 samples, giving a prevalence rate of 14%. Three standard methods used were Combine disc diffusion test (CDDT), Double disc synergy test (DDST), and Enzyme MIC strip method. Among the three methods CDDT method showed maximum sensitivity, and the Ezy MIC strip method was found to be least sensitive. Multi-drug resistant profiles of ESBL-positive isolates were also observed against Amikacin, Cefuroxime, Cefixime, Ampicillin...