Climate change is a pressing global issue that is caused by the consumption of fossil fuels, which releases greenhouse gases into the atmosphere. Cell factories are biological systems that are engineered to produce a wide range of products, such as biofuels, bioplastics, and pharmaceuticals which can play a crucial part in reducing the dependency on fossil fuels. Additionally, cell factories can be made more efficient and sustainable by using advanced technologies such as metabolic engineering and synthetic biology. This thesis aimed to expand the genetic toolbox of Pseudomonas taiwanensis...