Artificial insemination increases the genetic potential for production. Reproductive failure is attributable to semen quality. Various factors affect the sperm quality in cryopreserved semen resulting in infertility. Semen analysis methods including zona binding assay are used to assess the semen quality. However, still the fertilization is not fully assured. Transcripts in spermatozoa play roles in sperm function and early embryonic development. Male fertility markers are associated with sperm motility, axoneme migration, flagellar movement and regulation, sperm energy, etc. which in turn...