This book starts with a short introduction on Aegilops speltoides species, the DREB genes and their relevance. Then the isolation and characterization of the DRF1 gene in several accessions is described. Further, the complete gene sequence in three accessions was analyzed by several computational tools, with particular attention to the region coding for the AP2 domain, responsible for DNA recognition and binding. A pseudogene was also identified and analyzed. Besides, unexpected, unrelated fragments obtained during the amplification of the gene were also analyzed. Several sequences of a part...