Genetic transformation studies were carried out to standardize a protocol for insect resistance gene (cry IAb) transfer in cabbage using Agrobacterium-mediated gene transfer technique. Agrobacterium tumefaciens strain containing npt-II and cryIAb genes in binary vector pBin Bt1 was used for genetic transformation. Both hypocotyls and cotyledon explants were used for transformation studies. Hypocotyl explants showed better shoot regeneration as compared to cotyledon. For shoot regeneration from cotyledon and hypocotyl explants the best medium was found to be MS + 2.0 mg/l BAP + 0.25 mg/l IAA...
Genetic transformation studies were carried out to standardize a protocol for insect resistance gene (cry IAb) transfer in cabbage using Agrobacterium...