Reduced compounds can be produced using metabolically engineered microbes, often relying on redox-cofactor-dependent biocatalysis. The prerequisite for outstanding performance requires a basic understanding of the redox metabolism of the production host, a simple redox chassis, and a target molecule for which a metabolic pathway exists. This thesis addresses these prerequisites using the obligate aerobe Pseudomonas taiwanensis VLB120.The essentiality of the three NADH dehydrogenases of the respiratory chain of P. taiwanensis VLB120 was investigated. A mutant lacking all three NADH...
Reduced compounds can be produced using metabolically engineered microbes, often relying on redox-cofactor-dependent biocatalysis. The prerequisite fo...
