SHAFEEQ, Ibrahim Hamdi, TEMEL, Yusuf, ÇiFTÇi, Mehmet
In this study, glucose 6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) which is an importance enzyme for the carbohydrate metabolism, was purified from quail's erythrocyte and characterized. The purification was performed by preparation of hemolysate and 2', 5'-ADP Sepharose-4B affinity chromatography. G6PD from quail's erythrocyte was obtained with a yield of 77.17% having a specific activity of 60.40EU/mg. protein. The overall purification fold was around 4137. The characterization studies were showed: the stable pH value of enzyme to be 7.5 in Tris-HCl buffer, optimum pH value to be 8.0 in...
In this study, glucose 6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) which is an importance enzyme for the carbohydrate metabolism, was purified from ...
