Trace elements play an important role in the complex metabolic pathways in human system and their deficiency or in excess may cause disease. Many people all over the world including Pakistan are suffering from urolithiasis i.e. a disease related to urinary stones. The aim of this research study was to develop methods for calibrating the laboratory made standard of urinary calculi, blood and urine against which element were estimated and to see their role in urolithiasis. The work was carried out in such a way so as to classify the estimation of elements in patients and healthy persons.A...