Foot and mouth disease is caused by Apthovirus, family Picornaviridae. In this study, RT-PCR base detection of FMDV for primary and serotype specific diagnosis was developed and sequence analysis was done for molecular epidemiology. Different types of clinically positive samples of FMD were collected from the field which includes swab samples from the mouth and hoff vesicles, serum, plasma, saliva and milk samples from cattle, buffalo and goats. RT-PCR based identification and optimization was done with universal primer sets (P1 / P2 and F1 / F2) with expected band of 216bps and 328bps. PCR...