TMCd1 is a stress inducible metallothionein (MT)gene that is expressed in Tetrahymena tropicalis lahorensis under cadmium metal stress. In the present study TMCd1 gene was expressed in E.coli and physiological importance of this Tetrahymena MT protein was evaluated. This stress inducible TMCd1 gene encodes 471 nucleotides, with TAA encoding glutamine (Gln) and TGA codes for a stop codon. To get the expression of full length protein the TMCd1 gene was mutated by site directed mutagenesis and TAA (at position 116) was converted to CAA (Gln). The MutTMCd1 gene was cloned in pET-41a expression...