The Ocimum sanctum plant, a native to tropical Asia, is a member of the Lamiaceae (mint) family, . Ocimum sanctum is also known as TULSI , which means The incomparable one . It is also called as HOLY BASIL .It has great health implications. It has been used in several ancient systems of medicines including Ayurveda, Greek, Roman, and Siddha for thousands of years. Throughout Indo-Pak subcontinent, Tulsi is considered "The Queen of Herbs" and is revered as a sacred plant infused with healing powers.

This research work includes the nutritional value of mung and mash bean of Pakistan, which are consumed as food and play significant role in human nutritional needs. The beans of mung and mash were used to prepare protein isolates where defatted beans flour was used as the starting material. Through this method 54% and 33% of protein isolates were made (from mung and mash varieties). The prepared protein isolates can be used in food industry. Mung bean and mash have high protein content (with acceptable functional properties), which makes it promising protein source in food application....

The present piece of work was an effort to find and isolate the bioactive compounds not only in naturally grown but also in in vitro grown cell and tissue lines of Mako under controlled environment and adjustable PGRs.The study of natural product s profile of tissue cultured plants using GC-MS, is a unique idea,adopted during the present work, which may lead to the production and identification of many novel bioactive compounds.