Reproductive efficiency is the primary factor affecting productivity of farm animals. Among the various causes of subfertility, anoestrus is the major reproductive disorders (Pandya et al., 1989). Prolonged postpartum acyclicity and anestrum are major sources of economic loss to buffalo breeders (El-Wishy, 2007). Postpartum ovarian activity and subsequent pregnancy may be affected by several factors such as breed, nutrition plan, milk yield, suckling, uterine involution, season of calving (Arya and Madan, 2001; Baruselli et al., 2001; Campo et al., 2002). The present study was conducted to...