The objective of the present investigation was to establish in vitro culture and plant regeneration methods from leaf base and rhizome bud explants of Z. officinale. The MS medium with 1.0 mg/l 2, 4-D proved to be the best for callus induction from leaf base explants. Shoot regeneration was achieved after subculturing the calli in different media formulation and 8.0 mg/l BA with 0.2 mg/l 2, 4-D was found to be the best for multiple shoot regeneration from callus through organogenesis. MS medium supplemented with 5.0 mg/l NAA was the best formulation for successful culture establishment as...