Nishinda (Vitex negundo) leaves, stem barks and roots were sequentially extracted with organic solvents Pet spt, EtOAc, acetone and MeOH. Efficacy of these extracts was determined against adult and different larval instars of three strains of T. castaneum using residual film method. The degree of toxicity in the order was leavesstem barksroots. The LD50 values for first through fourth instar larvae were more or less similar whereas, LD50 for fifth instar larva and adults were the lowest. The degree of toxicity according to solvents was MeOHet spt.acetoneEtOAc. The susceptibility order...

The objective of the present investigation was to establish in vitro culture and plant regeneration methods from leaf base and rhizome bud explants of Z. officinale. The MS medium with 1.0 mg/l 2, 4-D proved to be the best for callus induction from leaf base explants. Shoot regeneration was achieved after subculturing the calli in different media formulation and 8.0 mg/l BA with 0.2 mg/l 2, 4-D was found to be the best for multiple shoot regeneration from callus through organogenesis. MS medium supplemented with 5.0 mg/l NAA was the best formulation for successful culture establishment as...

Different aspects of micropropagation through meristem culture for the production of virus indexed source plants, callus induction followed by subsequent plant regeneration, in vitro tuberization and field evaluation of the in vitro regenerated plants were studied on four commercial cultivars of potato (Solanum tuberosum L.) viz., Diamant, Cardinal, Shilbilati and Lalpakri. Rooted plantlets of four potato cultivars obtained directly through meristem culture and indirectly via callus phase were gradually acclimatized and successfully established in the field. Visual evaluation of the...