Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost.

However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells.

Based on the...