The aim was to develop and validate high performance liquid chromatography assay for rapid determination of Fampridine which should offer simplicity, reproducibility, selectivity, sensitivity, and accuracy of the assay method which should be suitable for routine analysis. Developed method has been successfully applied for the analysis of tablets and can be used for the routine analysis of formulations containing above drug without any alteration in the assay. The superiority of the method is common chromatographic conditions adopted for formulation. The simplicity, specificity, selectivity,...