This volume constitutes the refereed proceedings of the Second International Conference on Research in Smart Cards, E-smart 2001, held in Cannes, France, in September 2001. The 20 revised full papers presented were carefully reviewed and selected from 38 submissions. Among the topics addressed are biometrics, cryptography and electronic signatures on smart card security, formal methods for smart card evaluation and certification, architectures for multi-applications and secure open platforms, middleware for smart cards and novel applications of smart cards.