The identification and quantification of carbohydrate content in aqueous solutions and biological systems are of great interest and importance. The polar nature and large sizes of some carbohydrates can cause difficulties in identification and separation processes. Problems include long retention times and degradation during separation procedures, such as gas chromatography (GC). This project has developed methodology for an optimal quantification and identification procedure. We have derivatized carbohydrates to improve detection limits and resolution in standard GC and gas chromatography...