Pseudomonas putida KT2440 is a biosafety strain, which has retained its capability to survive and function in the environment. Its broad metabolic capability to degrade a broad spectrum of aromatic compounds and still grow at temperatures as low as 5 C makes it a good candidate as a model organism to study genome wide responses to changing growth conditions which mimic environmental changes in natural habitats. In this study a functional genomics approach was used to examine the stress response of P. putida KT2440 wild type and five cold sensitive Tn5 mutants (with plasposons inserted in...