Charophyte research of 32 districts had been documented on extant charophytes (Characeae) from 1929-2010 within Bangladesh. In all, 41 taxa of charophytes were found, represented by five genera. These are Chara(19), Nitella(18) while Lychnothamnus, Lamprothamnium and Nitellopsis were reported by single taxa each. Two Nitella had been identified as new species in 2004, collected from rice field which were not yet described in home and abroad.These are: Nitella rajshahiensis Naz., Zaman., ex Diba sp. nov. and Nitella zamanii Naz ex Diba sp. nov. and first time these species had been included in...