Gene pyramiding approach was used to
produce transgenic cotton expressing two Bt genes for
sustainable resistance. The vector construct pK2Ac
contained Bt genes and nptII gene both driven by CaMV35S
promoter, confers resistance to
Kanamycin.Sonication-assisted Agrobacterium-mediated
transformation system was used. Heliothis armigera 2nd
instar larval mortality ranged 75-100% and Bt protein was
quantified in the leaves of transgenic plants. Segregation
showed inheritance pattern 3:1...