Bio-Pots produced in this study were from primary pulp (SFI), secondary pulp (newspapers) and pulp mixture (SFI and newspapers). Bio-Pot formed using mould and oven-dried for 3 hours. Petai Belalang seedlings were planted in all three types of Bio-Pot and carried out at location above ground and underground for 60 days. The objectives of this study were to determine the weight loss of Bio-Pot, to determine the root penetration and the height rate of Petai Belalang seedlings.