Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium...